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Gp100 Antibody Cocktail / PMEL17

The gp100 molecule is a 100kDa glycosylated protein that is cleaved into a small (26kDa) carboxy-terminal fragment and a larger amino- terminal section (60-64 kDa), which is subsequently cleaved to generate 26kDa and 34-38kDa.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT (1)

UniProt

P40967

Host

Mouse

Reactivity

Human

Immunogen

Extract of pigmented melanoma metastases from lymph nodes (HMB45) and recombinant human protein (PMEL/783) were used as the immunogen for the gp100 antibody cocktail.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

HMB45 + PMEL/783

Applications

IHC-P, WB

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the gp100 antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This gp100 antibody cocktail is available for research use only.

Storage Conditions

Store the gp100 antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cytoplasmic

Image Legend

IHC: Formalin-fixed, paraffin-embedded human melanoma stained with gp100 antibody cocktail (HMB45 + PMEL/783) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

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