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ACTA2 Antibody Cocktail / Alpha Actin

Actin is a major component of the cytoskeleton and is present in most cell types. It is highly specific to actin from smooth muscles. This mAb does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.

Product Specifications

Specifications

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

UniProt

P62736

Host

Mouse

Reactivity

Human, Rat

Immunogen

An N-terminal decapeptide of the alpha smooth muscle isoform of Actin (1A4) and recombinant full-length human protein (ACTA2/791) were used as the immunogen for the ACTA2 antibody cocktail.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

1A4 + ACTA2/791

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

Reconstitution

Store the ACTA2 antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This ACTA2 antibody cocktail is available for research use only.

Storage Conditions

Store the ACTA2 antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Product Datasheet

https://www.nsjbio.com/tds-pdf/acta2-antibody-cocktail-alpha-actin-1a4-acta2791-v2838

Formulation

Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

CAS Number

9007-83-4

Location

Cytoplasmic

Image Legend

IHC: Formalin-fixed, paraffin-embedded human leiomyosarcoma stained with ACTA2 antibody cocktail (clone 1A4 + ACTA2/791) .

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