Cyclin D1 Antibody PE Conjugate
Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60% of mantle cell lymphomas (MCL) contain a t (11; 14) (q13; q32) translocation resulting in over-expression of cyclin D1. This antibody is useful in identifying mantle cell lymphomas (cyclin D1 positive) from CLL/SLL and follicular lymphomas (cyclin D1 negative) . Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow Cytometry: 5ul per test per one million cells in 100ul
UniProt
P24385
Host
Mouse
Reactivity
Human
Immunogen
Human recombinant full length protein was used as the immunogen for the Cyclin D1 antibody.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
SPM587
Applications
FACS
Purity
Protein G affinity chromatography
Format
PE Conjugate
Buffer
500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the Cyclin D1 antibody at 2-8oC. Conjugate is light sensitive, store in the dark.
Limitations
This Cyclin D1 antibody is available for research use only.
Storage Conditions
Store the Cyclin D1 antibody at 2-8°C. Conjugate is light sensitive, store in the dark.
Formulation
500 ul at 0.1 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the Cyclin D1 antibody should be determined by the researcher.
Location
Nuclear
Image Legend
Flow Cytometry testing of Jurkat cells. Black: cells alone; Green: isotype control; Red: PE-labeled Cyclin D1 antibody (SPM587) .
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