UACA Antibody
UACA (Uveal Autoantigen with Coiled-coil domains and Ankyrin repeats) is a 1,416 amino acid nuclear membrane protein. It was originally identified as an autoantigen in patients with panuveitis, a characteristic of Vogt-Koyanagi-Harada disease, and in patients with Graves' disease. UACA was also later identified as Nucling, an mRNA differentially expressed in F9 embryonal carcinoma cells during cardiac muscle differentiation. UACA appears to function as a pro-apoptotic protein that recruits the apaf-1-pro-caspase-9 complex for the induction of apoptosis to mediate the cell-death pathway.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 0.5-1 µg/mL, Western blot: 0.5-1 µg/mL
UniProt
Q9BZF9
Host
Mouse
Reactivity
Human, Mouse
Immunogen
Nuclei of myeloid leukemia biopsy cells were used as the immunogen for the UACA antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
AE-5
Applications
FACS, IF, WB
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the UACA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This UACA antibody is available for research use only.
Storage Conditions
Store the UACA antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the UACA antibody should be determined by the researcher.
Location
Nuclear membrane
Image Legend
Western blot of A549 cell lysate using UACA antibody (AE-5)
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