TRAcP Antibody / TRAP / ACP5

It recognizes a protein of 35kDa, which is identified as tartrate-resistant acid phosphatase (TRAcP) . It exists as two isoforms (5a and 5b) . This mAb reacts with both the isoforms. Serum TRAcP 5a is secreted by macrophages and dendritic cells and increased in many patients of rheumatoid arthritis. Serum TRAcP 5b is produced from osteoclasts and elevated during bone resorption. TRAcP is an iron containing glycoprotein, which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is the most basic of the acid phosphatases and is the only form not inhibited by L (+) -tartrate. TRAcP is synthesized as a latent proenzyme and is activated by proteolytic cleavage and reduction. Normally, TRAcP is highly expressed by osteoclasts, activated macrophages, neurons and endometrium during pregnancy. Expression of TRAcP is increased in certain pathological conditions such as Leukemic Reticuloendotheliosis (Hairy Cell Leukemia), Gaucher's Disease, HIV-induced Encephalopathy, Osteoclastoma and in osteoporosis and metabolic bone diseases. Anti-TRAcP antibody labels the cells of Hairy Cell Leukemia (HCL) with a high degree of sensitivity and specificity. Other cells stained with this antibody are tissue macrophages and osteoclasts.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT

UniProt

P13686

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant full-length human protein was used as the immunogen for the TRAcP antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

ACP5/1070

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the TRAcP antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This TRAcP antibody is available for research use only.

Storage Conditions

Store the TRAcP antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the TRAcP antibody should be determined by the researcher.1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.