PECAM-1 Antibody / CD31
CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 mAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL
UniProt
P16284
Host
Mouse
Reactivity
Human
Immunogen
Stimulated human leukocytes (Workshop VI) were used as the immunogen for the PECAM-1 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
158-2B3
Applications
FACS, IF
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Store the PECAM-1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This PECAM-1 antibody is available for research use only.
Storage Conditions
Store the PECAM-1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
Optimal dilution of the PECAM-1 antibody should be determined by the researcher.
Location
Cell surface and cytoplasm of endothelial cells
Image Legend
Immunofluorescent staining of PFA-fixed human Jurkat cells with PECAM-1 antibody (green, clone 158-2B3) and Reddot nuclear counterstain (red) .
Documents
Frequently Asked Questions
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