CD31 Antibody
CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 mAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/10^6 cells, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P16284
Host
Mouse
Reactivity
Human
Immunogen
Recombinant human protein was used as the immunogen for the CD31 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
C31.10
Applications
FACS, WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the CD31 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This CD31 antibody is available for research use only.
Storage Conditions
Store the CD31 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Cell surface and cytoplasm of endothelial cells
Image Legend
Flow cytometry test of Jurkat cells. Black: cells alone; Grey: isotype control; Green: AF488-labeled CD31 antibody (C31.10) .
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