PCNA Antibody
Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA) . It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This mAb is excellent for multiple applications.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/10^6 cells, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 0.25-0.5 µg/mL for 30 min at RT
UniProt
P12004
Host
Mouse
Reactivity
Human
Immunogen
Recombinant full length human protein was used as the immunogen for the PCNA antibody.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
PCNA/694
Applications
FACS, WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the PCNA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This PCNA antibody is available for research use only.
Storage Conditions
Store the PCNA antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Predominantly nuclear, some cytoplasmic
Image Legend
Flow cytometry testing of PFA-fixed human HeLa cells with PCNA antibody (clone PCNA/694) ; Red=isotype control, Blue= PCNA antibody.
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