NuMA Antibody
Recognizes a phosphorylated protein of 228kDa, identified as nuclear mitotic apparatus protein (NuMA) . NuMA is intra-nuclear protein and present in nucleus during interphase. At the onset of mitosis, it redistributes from the nucleus to two centrosomal structures that later will become part of the mitotic spindle pole. After anaphase, the protein redistributes from the spindle polar region into reforming nucleus. NuMA is an essential protein during mitosis for the terminal phases of chromosome separation and/or nuclear reassembly. Recently a study shows that NuMA is cleaved to a 180 to 200kDa during apoptosis. Chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 has been detected in patients with acute promyelocytic leukemia.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
Q14980
Host
Mouse
Reactivity
Human
Immunogen
Colon carcinoma 174T cells were used as the immunogen for the NuMA antibody.
Clonality
Monoclonal
Isotype
IgM k
Clone
SPM300
Applications
IHC-P
Purity
PEG precipitation
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the NuMA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This NuMA antibody is available for research use only.
Storage Conditions
Store the NuMA antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Location
Nuclear
Image Legend
IHC analysis of formalin-fixed, paraffin-embedded human tonsil stained with NuMA antibody (clone SPM300) .
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