NuMA Antibody

Recognizes a phosphorylated protein of 228kDa, identified as nuclear mitotic apparatus protein (NuMA) . Its epitope is resistant to phosphatases. NuMA is intra-nuclear protein and present in nucleus during interphase. At the onset of mitosis, it redistributes from the nucleus to two centrosomal structures that later will become part of the mitotic spindle pole. After anaphase, the protein redistributes from the spindle polar region into reforming nucleus. NuMA is an essential protein during mitosis for the terminal phases of chromosome separation and/or nuclear reassembly. Recently a study shows that NuMA is cleaved to a 180 to 200kDa during apoptosis. Chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 has been detected in patients with acute promyelocytic leukemia.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

Q14980

Host

Mouse

Reactivity

Human

Immunogen

Colon carcinoma 174T cells were used as the immunogen for the NuMA antibody.

Clonality

Monoclonal

Isotype

IgM k

Clone

A73-B/D12

Applications

IHC-P

Purity

PEG precipitation

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the NuMA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This NuMA antibody is available for research use only.

Storage Conditions

Store the NuMA antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the NuMA antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.