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Nucleolin Antibody

Recognizes a protein of ~76kDa, which is identified as Nucleolin (NCL) . It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.

Product Specifications

CAS Number

9007-83-4

Specifications

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

UniProt

P19338

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant human protein was used as the immunogen for the Nucleolin antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

NCL/902

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

Reconstitution

Store the Nucleolin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Nucleolin antibody is available for research use only.

Storage Conditions

Store the Nucleolin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

Location

Nucleoli

Image Legend

IHC: Formalin-fixed, paraffin-embedded human tonsil stained with Nucleolin antibody.

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