CD56 Antibody / NCAM / Extracellular domain

This mAb reacts with an extracellular domain (close to transmembrane) of CD56/NCAM. Three isoforms of neural cell adhesion molecule (NCAM) are produced by differential splicing of the RNA transcript from a single gene. The 135kDa isoform is the basic molecule, which is glycosylated or sialylated to produce the mature species. Anti-CD56 recognizes two proteins of the neural cell adhesion molecule, the basic molecule expressed on most neuroectodermally derived tissues and neoplasms (e.g. retinoblastoma, medulloblastomas, astrocytomas, neuroblastomas, and small cell carcinomas) . It is also expressed on some mesodermally derived tumors (rhabdomyosarcoma) . Anti-CD56 plays an important role in the diagnosis of nodal and nasal NK/T-cell lymphomas.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT (1), Prediluted IHC only format: incubate for 30 min at RT (2)

UniProt

P13591

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human protein was used as the immunogen for the CD56 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

NCAM1/795

Applications

WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the CD56 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This CD56 antibody is available for research use only.

Storage Conditions

Store the CD56 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cell surface, cytoplasmic

Image Legend

Western blot analysis of human HCT116 cell lysate using CD56 antibody. Predicted molecular weight: ~110 kDa (soluble fragment), ~120/125 kDa (GPI-anchored), 140/180 kDa (transmembrane isoforms) .