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MUC5AC Antibody

This mAb recognizes the peptide core of gastric mucin M1 (recently identified as Mucin 5AC) . Its epitope is destroyed by beta-mercaptoethanol but not by periodate treatment. This mucin is present in primary ovarian mucinous cancer but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. Together with a panel of antibodies, Anti-MUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification of intestinal metaplasia as well as in the identification of pancreatic carcinoma and pre-cancerous changes vs. normal pancreas.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA: 1-5 µg/mL for coating (order BSA/sodium azide-free format)

UniProt

P98088

Host

Mouse

Reactivity

Human

Immunogen

An M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le (a-b) patient was used as the immunogen for the MUC5AC antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

58M1

Applications

ELISA

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the MUC5AC antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This MUC5AC antibody is available for research use only.

Storage Conditions

Store the MUC5AC antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the MUC5AC antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

SDS-PAGE analysis of purified, BSA-free MUC5AC antibody (clone 58M1) as confirmation of integrity and purity.

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