MUC1 Antibody / Mucin-1 / EMA
In Western blotting, it recognizes proteins in MW range of 265-400 kDa, identified as different glycoforms of MUC1/Mucin-1/Epithelial Marker Antigen/EMA. The alpha subunit has cell adhesive properties. It can act both as an adhesion and an anti-adhesion protein. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain, which is involved in cell signaling, through phosphorylations and protein-protein interactions. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinoma tissues. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.
Product Specifications
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 1-2 µg/mL
UniProt
P15941
Host
Mouse
Reactivity
Human
Immunogen
Human milk-fat globule membranes (HMFGM) was used as the immunogen for the MUC1 antibody.
Clonality
Monoclonal
Isotype
IgG2a λ
Clone
MUC1/520
Applications
IHC-P, WB
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the MUC1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This MUC1 antibody is available for research use only.
Storage Conditions
Store the MUC1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Product Datasheet
https://www.nsjbio.com/tds-pdf/muc1-antibody-mucin-1-ema-muc1520-v2725
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
CAS Number
9007-83-4
Location
Cytoplasmic and cell surface
Image Legend
Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with EMA antibody (MUC1/520) .
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
Recombinant Schizosaccharomyces pombe Mediator of RNA polymerase II transcription subunit 21 (med21)
Recombinant Schizosaccharomyces pombe Mediator of RNA polymerase II transcription subunit 21 (med21)
Recombinant Schizosaccharomyces pombe Mediator of RNA polymerase II transcription subunit 21 (med21)
Recombinant Schizosaccharomyces pombe Mediator of RNA polymerase II transcription subunit 21 (med21)
Recombinant Schizosaccharomyces pombe Mediator of RNA polymerase II transcription subunit 21 (med21)
