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MUC1 Antibody / Mucin-1 / EMA

In Western blotting, it recognizes proteins in MW range of 265-400 kDa, identified as different glycoforms of MUC1/Mucin-1/Epithelial Marker Antigen/EMA. The alpha subunit has cell adhesive properties. It can act both as an adhesion and an anti-adhesion protein. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain, which is involved in cell signaling, through phosphorylations and protein-protein interactions. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinoma tissues. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 1-2 µg/mL

UniProt

P15941

Host

Mouse

Reactivity

Human

Immunogen

Human milk-fat globule membranes (HMFGM) was used as the immunogen for the MUC1 antibody.

Clonality

Monoclonal

Isotype

IgG2a λ

Clone

MUC1/520

Applications

IHC-P, WB

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the MUC1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This MUC1 antibody is available for research use only.

Storage Conditions

Store the MUC1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cytoplasmic and cell surface

Image Legend

Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with EMA antibody (MUC1/520) .

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