CD99 Antibody
CD99 is involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. [UniProt]
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 1-2 µg/mL
UniProt
P14209
Host
Mouse
Reactivity
Human, Rat
Immunogen
Purified E-rosette forming cells from human peripheral blood lymphocytes were used as the immunogen for the CD99 antibody.
Clonality
Monoclonal
Isotype
IgM k
Clone
HO36-1.1
Applications
FACS, IF, IHC-P, WB
Purity
PEG precipitation
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the CD99 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This CD99 antibody is available for research use only.
Storage Conditions
Store the CD99 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Cell surface
Image Legend
IHC: Formalin-fixed, paraffin-embedded human testicular carcinoma stained with CD99 antibody (HO36-1.1) .
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