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Lactadherin Antibody

Recognizes a protein of 40-45kDa, identified as human milk fat globule membrane protein (HMFG), Lactadherin or MFG-EA. HMFG is present on normal human breast epithelial cells and cell lines derived from breast carcinomas, as well as to the outer surface of the human milk fat globule. HMFG is considered as a differentiation marker. It is useful as specific breast epithelial marker and can also provide a tool to study the role of the cell surface in normal and neoplastic mammary development.

Product Specifications

CAS Number

9007-83-4

Specifications

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

UniProt

Q08431

Host

Mouse

Reactivity

Human

Immunogen

A delipidated human milk fat globule membrane preparation was used as the immunogen for the Lactadherin antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

EDM45

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

Reconstitution

Store the Lactadherin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Lactadherin antibody is available for research use only.

Storage Conditions

Store the Lactadherin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Product Datasheet

https://www.nsjbio.com/tds-pdf/lactadherin-antibody-edm45-v2705

Formulation

Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

Location

Cell surface & cytoplasmic

Image Legend

IHC analysis of formalin-fixed, paraffin-embedded human breast carcinoma stained with Lactadherin antibody (EDM45)

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