Lactadherin Antibody
Recognizes a protein of 40-45kDa, identified as human milk fat globule membrane protein (HMFG), Lactadherin or MFG-EA. HMFG is present on normal human breast epithelial cells and cell lines derived from breast carcinomas, as well as to the outer surface of the human milk fat globule. HMFG is considered as a differentiation marker. It is useful as specific breast epithelial marker and can also provide a tool to study the role of the cell surface in normal and neoplastic mammary development.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT
UniProt
Q08431
Host
Mouse
Reactivity
Human
Immunogen
A delipidated human milk fat globule membrane preparation was used as the immunogen for the Lactadherin antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
EDM45
Applications
FACS, IF, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the Lactadherin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Lactadherin antibody is available for research use only.
Storage Conditions
Store the Lactadherin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Cell surface & cytoplasmic
Image Legend
IHC analysis of formalin-fixed, paraffin-embedded human breast carcinoma stained with Lactadherin antibody (EDM45)
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