EpCAM Antibody

EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM) . It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-3 µg/mL

UniProt

P16422

Host

Mouse

Reactivity

Human

Immunogen

Small cell lung carcinoma cells were used as the immunogen.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

SPM134

Applications

FACS, IF

Purity

Protein G affinity chromatography

Format

CF488 Conjugate

Buffer

500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the EpCAM antibody at 2-8oC, protected from light.

Limitations

This EpCAM antibody is available for research use only.

Storage Conditions

Store the EpCAM antibody at 2-8°C, protected from light.

Formulation

500 ul at 0.1 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the EpCAM antibody should be determined by the researcher.

Location

Cell surface

Image Legend

Flow cytometry testing of PFA-fixed human MCF7 cells with unlabeled anti-EpCAM antibody (clone SPM134) + CF488-labeled secondary; Red=isotype control, Blue= anti-EpCAM antibody.