Keratin 18 Antibody
This mAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/10^6 cells, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P05783
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
A cytoskeletal preparation from A431 cells was used as the immunogen for the Keratin 18 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
C-04 or Ks18.04
Applications
FACS, WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the Keratin 18 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Keratin 18 antibody is available for research use only.
Storage Conditions
Store the Keratin 18 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Cytoplasmic
Image Legend
Western blot testing of human intestine and stomach tissue lysate with Keratin 18 antibody (clone C-04) . Expected molecular weight: 46-50 kDa.
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