CK18 Antibody / Cytokeratin 18
This mAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It is reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT (1)
UniProt
P05783
Host
Mouse
Reactivity
Human
Immunogen
Recombinant human KRT18 protein was used as the immunogen for the CK18 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
KRT18/836
Applications
IHC-P, IF, WB
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the CK18 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This CK18 antibody is available for research use only.
Storage Conditions
Store the CK18 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Cytoplasmic
Image Legend
Immunofluorescent staining of human HeLa cells with CK18 antibody (clone KRT18/836, green) and Reddot nuclear stain (red) .
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