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Cytokeratin 7 Antibody

Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. Antibody to cytokeratin is useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-) .

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P08729

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human KRT7 protein was used as the immunogen for the Cytokeratin 7 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

KRT7/903

Applications

FACS, IF, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the Cytokeratin 7 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Cytokeratin 7 antibody is available for research use only.

Storage Conditions

Store the Cytokeratin 7 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Location

Cytoplasmic

Image Legend

Immunofluorescent staining of methanol fixed human HeLa cells with Cytokeratin 7 antibody (clone KRT7/903, green) and Reddot nuclear stain (red) .

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