Keratin 7 Antibody
It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This mAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This mAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-) .
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P08729
Host
Mouse
Reactivity
Human
Immunogen
OTN 11 cells (ovarian carcinoma cell line) were used as the immunogen for the Keratin 7 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
SPM270
Applications
WB, FACS, IF, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the Keratin 7 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Keratin 7 antibody is available for research use only.
Storage Conditions
Store the Keratin 7 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Cytoplasmic
Image Legend
Immunofluorescent staining of permeabilized human HeLa cells with Keratin 7 antibody (clone SPM270, green) and Reddot nuclear stain (red) .
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