Cytokeratin 7 Antibody

CK7 blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7) . Expressed in cultured epidermal, bronchial and mesothelial cells. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P08729

Host

Mouse

Reactivity

Human

Immunogen

OTN 11 cells (ovarian carcinoma cell line) were used as the immunogen for the Cytokeratin 7 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

OV-TL12/30

Applications

WB, FACS, IF, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the Cytokeratin 7 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Cytokeratin 7 antibody is available for research use only.

Storage Conditions

Store the Cytokeratin 7 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Cytokeratin 7 antibody

Location

Cytoplasmic

Image Legend

IHC: Formalin-fixed, paraffin-embedded ovarian carcinoma stained with Cytokeratin 7 antibody (clone OV-TL12/30) . HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.