C-Kit Antibody

This mAb recognizes a protein of 145kDa, identified as CD117/p145kit/c-Kit. It is found on a wide variety of tumor cells including follicular and papillary carcinoma of thyroid, adenocarcinomas from endometrium, lung, ovary, pancreas, and breast as well as malignant melanoma, endodermal sinus tumor, and small cell carcinoma. However, anti-CD117/c-Kit has been particularly useful in differentiating gastrointestinal stromal tumors from Kaposi's sarcoma, tumors of smooth muscle origin, fibromatosis, and neural tumors of the GI tract. Anti-CD117/c-Kit is also useful in recognizing myeloblasts in bone marrow biopsy and clot section.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 2-4 µg/mL

UniProt

P10721

Host

Mouse

Reactivity

Human

Immunogen

A recombinant human fragment around amino acids 100-300 was used as the immunogen for the c-Kit antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

KIT/983

Applications

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the c-Kit antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This c-Kit antibody is available for research use only.

Storage Conditions

Store the c-Kit antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the c-Kit antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min