CD18 Antibody
It recognizes a transmembrane glycoprotein of 95kDa, identified as CD18 or integrin -2 (Workshop III) . It complexes non-covalently with either L, M, or X integrin (CD11a, b, or c) to form the heterodimers, LFA-1, Mac-1, and p150,95, respectively. LFA-1 is the receptor for three members of the Ig supergene family of proteins, ICAM-1 (CD54), ICAM-2 (CD102), and Mac-1 and p150,95 bind to ICAM-1, fibrinogen, and iC3b. ICAM-3 (CD50) . CD18/CD11 heterodimeric molecules are involved with cell/cell and cell/extracellular adhesion in immune and inflammatory responses. This mAb blocks these cellular interactions.
Product Specifications
CAS Number
9007-83-4
Specifications
Functional studies (order BSA/sodium azide-free format), Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL
UniProt
P05107
Host
Mouse
Reactivity
Human
Immunogen
Stimulated human leukocytes were used as the immunogen for the CD18 antibody.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
68-5A5
Applications
Functional studies, FACS, IF
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the CD18 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This CD18 antibody is available for research use only.
Storage Conditions
Store the CD18 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the CD18 antibody should be determined by the researcher.
Location
Cell surface
Image Legend
SDS-PAGE Analysis of Purified, BSA-Free CD18 Antibody (clone 68-5A5) . Confirmation of Integrity and Purity of the Antibody.
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