Anti-IgG Antibody
Recognizes a protein of 75kDa, identified as gamma heavy chain of human immunoglobulins. Its epitope maps in CH2 domain of Fc region of IgG. It reacts with all sub-classes of gamma chain of human immunoglobulins. It does not cross-react with alpha (IgA), mu (IgM), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This mAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkins lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT
UniProt
P01857, P01859, P01860, P01861
Host
Mouse
Reactivity
Human
Immunogen
Purified polyclonal human Ig Gamma Chain was used as the immunogen for the Anti-IgG antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
B33/20
Applications
FACS, IF, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the Anti-IgG antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Anti-IgG antibody is available for research use only.
Storage Conditions
Store the Anti-IgG antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Cytoplasm, cell surface and secreted
Image Legend
IHC: Formalin-fixed, paraffin-embedded human tonsil stained with anti-IgG antibody (B33/20)
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