IDH1 Antibody
It recognizes a 45kDa protein, which is identified as isocitrate dehydrogenase (IDH1) . It belongs to the isocitrate and isopropylmalate dehydrogenases family. IDH1 catalyzes the third step of the citric acid cycle, which involves the oxidative decarboxylation of isocitrate, forming a-ketoglutarate and CO2 in a two-step reaction. The first step involves the oxidation of isocitrate to the intermediate oxalosuccinate, while the second step involves the production of a-ketoglutarate. During this process, either NADH or NADPH is produced along with CO2. Recently, an inactivating mutation of IDH1 has been implicated in glioblastoma. IDH1 appears to function as a tumor suppressor that, when mutationally inactivated, contributes to tumorigenesis in part through induction of the HIF-1 pathway.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Immunofluorescence: 1-2 µg/mL
UniProt
O75874
Host
Mouse
Reactivity
Human
Immunogen
A recombinant fragment (119 amino acid residues around aa 280-420) from the human protein was used as the immunogen for the IDH1 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
IDH1/1152
Applications
IHC-P, IF
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the IDH1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This IDH1 antibody is available for research use only.
Storage Conditions
Store the IDH1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Cytoplasmic & nuclear
Image Legend
IHC: Formalin-fixed, paraffin-embedded human colon carcinoma stained with IDH1 antibody (clone IDH1/1152) .
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