ICAM3 Antibody

Recognizes an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This mAb is excellent for staining of formalin/paraffin tissues.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P32942

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human protein was used as the immunogen for the ICAM3 antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

ICAM3/1019

Applications

WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the ICAM3 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This ICAM3 antibody is available for research use only.

Storage Conditions

Store the ICAM3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Membrane, cytoplasm

Image Legend

Western blot analysis of HL-60 cell lysate using ICAM3 antibody (ICAM3/1019) . Observed molecular weight: 60~120 kDa depending on glycosylation level.