ICAM3 Antibody
Recognizes an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This mAb is excellent for staining of formalin/paraffin tissues.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P32942
Host
Mouse
Reactivity
Human
Immunogen
Recombinant human protein was used as the immunogen for the ICAM3 antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
ICAM3/1019
Applications
WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the ICAM3 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This ICAM3 antibody is available for research use only.
Storage Conditions
Store the ICAM3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Membrane, cytoplasm
Image Legend
Western blot analysis of HL-60 cell lysate using ICAM3 antibody (ICAM3/1019) . Observed molecular weight: 60~120 kDa depending on glycosylation level.
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