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Anti-ICAM-3 Antibody

Recognizes the D1 domain of an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This mAb is excellent for staining of formalin/paraffin tissues.

Product Specifications

CAS Number

9007-83-4

Specifications

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

UniProt

P32942

Host

Mouse

Reactivity

Human

Immunogen

Stimulated human leukocytes were used as the immunogen for the anti-ICAM-3 antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

SPM505

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

Reconstitution

Store the anti-ICAM-3 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This anti-ICAM-3 antibody is available for research use only.

Storage Conditions

Store the anti-ICAM-3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

Location

Membrane, cytoplasm

Image Legend

Formalin-fixed, paraffin-embedded human tonsil stained with anti-ICAM-3 antibody (SPM505)

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