ICAM1 Antibody / CD54
Recognizes an 85-115kDa protein (variation with cell type), identified as intercellular adhesion molecule (ICAM-1) . It has 7 potential N-linked glycosylation sites. ICAM1 is a single chain glycoprotein of Ig supergene family, present on unstimulated endothelial cells (EC) and on a variety of other cell types including activated fibroblasts, EC, macrophages, and lymphocytes. ICAM1 mediates cell adhesion by binding to integrins CD11a/CD18 (leukocyte adhesion molecule, LFA-1) and to CD11b/CD18 (Mac-1) . This interaction enhances antigen-specific T-cell activation. ICAM1 also binds to CD43 and to Plasmodium falciparum infected RBCs and may also be related to progression and metastasis of tumors.
Product Specifications
CAS Number
9007-83-4
Specifications
Functional studies (order BSA/sodium azide-free format), Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 0.5-1 µg/mL
UniProt
P05362
Host
Mouse
Reactivity
Human
Immunogen
Human umbilical cord vein endothelial cells (HUVEC) were used as the immunogen for the ICAM1 antibody.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
F4-31C2
Applications
Functional studies, FACS, IF
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the ICAM1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This ICAM1 antibody is available for research use only.
Storage Conditions
Store the ICAM1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the ICAM1 antibody should be determined by the researcher.
Location
Cell surface
Image Legend
SDS-PAGE Analysis of Purified, BSA-Free ICAM1 Antibody (clone F4-31C2) . Confirmation of Integrity and Purity of the Antibody.
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