Tenascin C Antibody

In Western blotting, it reacts with two bands of ~MW of 210kDa and 300kDa, identified as two isoforms of Tenascin C. Specificity of this mAb is validated by sequential immunoprecipitation with a PAb against Tenascin C. Tenascin C is a multifunctional, disulfide-linked hexameric extracellular matrix glycoprotein expressed in association with mesenchymal epithelial interactions during development and in the neo-vasculature and stroma of undifferentiated tumors. In adults, it is restricted to certain epithelial-stromal interfaces and increases markedly in hyper-proliferative diseases and in stroma of many neoplasms, including gliomas, breast, squamous and lung carcinomas.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 2-4 µg/mL for 30 min at RT

UniProt

P24821

Host

Mouse

Reactivity

Human

Immunogen

Human breast carcinoma cells were used as the immunogen for the Tenascin C antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

T2H5

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the Tenascin C antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Tenascin C antibody is available for research use only.

Storage Conditions

Store the Tenascin C antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Connective tissue matrix

Image Legend

IHC: Formalin-fixed, paraffin-embedded human lung carcinoma stained with Tenascin C antibody (T2H5) at 4ug/ml. Antigen retrieval in 10mM Tris with 1mM EDTA, pH 9.0; ABC detection system with DAB Chromogen. Note staining of connective tissue.