HSP27 Antibody

9007-83-4

Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 1-2 µg/mL

P04792

Mouse

Human

Recombinant human HSPB1 protein was used as the immunogen for the HSP27 antibody.

Monoclonal

IgG1 k

HSPB1/774

FACS, IF, IHC-P, WB

Protein G affinity chromatography

Purified

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Store the HSP27 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

This HSP27 antibody is available for research use only.

Store the HSP27 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Predominantly cytoplasmic with some nuclear

Immunofluorescent staining of PFA-fixed human MCF7 cells with HSP27 antibody (clone HSPB1/774, green) and Reddot nuclear stain (red) .