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HLA-DRB1 Antibody (MHC II)

L243 monoclonal antibody

Product Specifications

CAS Number

9007-83-4

Specifications

Flow Cytometry: 1-2ug/million cells in 0.1ml, Immunofluorescence: 2-4 µg/mL, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)

UniProt

P01911

Host

Mouse

Reactivity

Human

Immunogen

Activated human peripheral blood mononuclear cells were used as the immunogen for the HLA-DRB1 antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

SPM289

Applications

FACS, IF, WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the HLA-DRB1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This HLA-DRB1 antibody is available for research use only.

Storage Conditions

Store the HLA-DRB1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cell surface

Image Legend

Immunofluorescent staining of human Raji cells with HLA-DRB1 antibody (clone SPM289, green) and Reddot nuclear stain (red) .

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