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HLA-DR Antibody (MHC II)

HLA-DR binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

UniProt

P01911

Host

Mouse

Reactivity

Human

Immunogen

Activated human peripheral blood mononuclear cells were used as the immunogen for the HLA-DR antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

LN3

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

Reconstitution

Store the HLA-DRB1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This HLA-DR antibody is available for research use only.

Storage Conditions

Store the HLA-DR antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

Location

Cytoplasmic, membranous

Image Legend

IHC staining of FFPE human tonsil tissue with HLA-DR antibody (clone LN3) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

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