HLA-DR Antibody (MHC II)
HLA-DR binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. [UniProt]
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-3 µg/mL, Western blot: 2-4 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P01911
Host
Mouse
Reactivity
Human
Immunogen
Activated human peripheral blood mononuclear cells were used as the immunogen for the HLA-DR antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
LN3
Applications
FACS, IF, WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the HLA-DRB1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This HLA-DR antibody is available for research use only.
Storage Conditions
Store the HLA-DR antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Cytoplasmic, membranous
Image Legend
Immunofluorescent staining of human Ramos cells with HLA-DR antibody (clone LN3, green) and Reddot nuclear stain (red) .
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