HLA-DR Antibody (MHC II)
This antibody detects a monomorphic general framework determinant of HLA-DR Class II antigen. It does not cross react with HLA-DP and HLA-DQ. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kDa alpha (heavy) chain and a 28kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense cytoplasmic staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL
UniProt
P01903
Host
Mouse
Reactivity
Human
Immunogen
Human peripheral blood leukocytes were used as the immunogen for the HLA-DR antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
169-1B5.2
Applications
FACS, IF
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the HLA-DR antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This HLA-DR antibody is available for research use only.
Storage Conditions
Store the HLA-DR antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the HLA-DR antibody should be determined by the researcher.
Location
Cell surface
Image Legend
SDS-PAGE Analysis of Purified, BSA-Free HLA-DR Antibody (clone 169-1B5.2) . Confirmation of Integrity and Purity of the Antibody.
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