HLA-DPB1 Antibody (MHC II)

Recognizes a non-polymorphic determinant of DP-MHC class II. MHC class II antigens are transmembrane glycoproteins of non-covalently linked alpha (33-35kDa) and beta (27-30kDa) chains. It reportedly reacts with B- & non-T, non-B cell lines but not with T- and myeloid cell lines and leukemias. Differential expression of MHC class II antigens on fetal and adult lymphocytes, malignant B cells appears to reflect the stage of cell differentiation which may be useful in the study of lymphoproliferative disorders.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA: 2-4 µg/mL for coating (order BSA/sodium azide-free format), Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL

UniProt

P04440

Host

Mouse

Reactivity

Human

Immunogen

Non-T, non-B human acute lymphoblastic leukemia REH6 cell line was used as the immunogen for the HLA-DPB1 antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

BraFB6

Applications

ELISA, FACS, IF

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the HLA-DPB1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This HLA-DPB1 antibody is available for research use only.

Storage Conditions

Store the HLA-DPB1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the HLA-DPB1 antibody should be determined by the researcher.1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.