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HLA-ABC Antibody (MHC I)

Reacts with a monomorphic determinant of human major histocompatibility (MHC) class I antigens (HLA-A, B and C) . Human MHC class I antigens are expressed constitutively on all nucleated cells lymphocytes such as lymphocytes, thymocytes, granulocytes, and bone marrow cells and are absent on erythrocytes. MHC class I antigens play a role in class I MHC-associated antigen presentation, inhibition of NK cell cytotoxicity, tumor surveillance, and tissue allotransplantation.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL

UniProt

P30443, P01889, P30499

Host

Mouse

Reactivity

Human, Mouse

Immunogen

Human peripheral blood leukocytes were used as the immunogen for the HLA-ABC antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

246-B8.E7

Applications

FACS, IF, WB

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the HLA-ABC antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This HLA-ABC antibody is available for research use only.

Storage Conditions

Store the HLA-ABC antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the HLA-ABC antibody should be determined by the researcher.

Location

Cell surface

Image Legend

Western blot testing of ThP-1 cell lysate with HLA-ABC antibody. Expected molecular weight of A/B/C: 40-41 kDa.

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