HLA-ABC Antibody (MHC I)
Reacts with a monomorphic determinant of human major histocompatibility (MHC) class I antigens (HLA-A, B and C) . Human MHC class I antigens are expressed constitutively on all nucleated cells lymphocytes such as lymphocytes, thymocytes, granulocytes, and bone marrow cells and are absent on erythrocytes. MHC class I antigens play a role in class I MHC-associated antigen presentation, inhibition of NK cell cytotoxicity, tumor surveillance, and tissue allotransplantation.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL
UniProt
P30443, P01889, P30499
Host
Mouse
Reactivity
Human, Mouse
Immunogen
Human peripheral blood leukocytes were used as the immunogen for the HLA-ABC antibody.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
246-B8.E7
Applications
FACS, IF, WB
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the HLA-ABC antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This HLA-ABC antibody is available for research use only.
Storage Conditions
Store the HLA-ABC antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the HLA-ABC antibody should be determined by the researcher.
Location
Cell surface
Image Legend
Western blot testing of ThP-1 cell lysate with HLA-ABC antibody. Expected molecular weight of A/B/C: 40-41 kDa.
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