Anti-Glycophorin A Antibody

Recognizes a sialoglycoprotein of 39kDa, identified as glycophorin A (GPA) . It is present on red blood cells (RBC) and erythroid precursor cells. It has been shown that glycophorin acts as the receptor for Sandei virus and parvovirus. Glycophorins A (GPA) and B (GPB), which are single, trans-membrane sialoglycoproteins. GPA is the carrier of blood group M and N specificities, while GPB accounts for S and U specificities. GPA and GPB provide the cells with a large mucin like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation.

Product Specifications

CAS Number

9007-83-4

Specifications

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

UniProt

P02724

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human GPA protein was used as the immunogen for the anti-Glycophorin A antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

SPM599

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

Reconstitution

Store the anti-Glycophorin A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This anti-Glycophorin A antibody is available for research use only.

Storage Conditions

Store the anti-Glycophorin A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

Applications Notes

Optimal dilution of the anti-Glycophorin A antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Location

Cytoplasmic, membranous

Image Legend

IHC testing of FFPE human placenta stained with anti-Glycophorin A antibody (clone SPM599) . Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.