Glycophorin A Antibody
Recognizes a sialoglycoprotein of 39kDa, identified as glycophorin A (GPA) . It is present on red blood cells (RBC) and erythroid precursor cells. It has been shown that glycophorin acts as the receptor for Sandei virus and parvovirus. Glycophorins A (GPA) and B (GPB), which are single, trans-membrane sialoglycoproteins. GPA is the carrier of blood group M and N specificities, while GPB accounts for S and U specificities. GPA and GPB provide the cells with a large mucin like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 1-2 µg/mL
UniProt
P02724
Host
Mouse
Reactivity
Human
Immunogen
Recombinant human GPA protein was used as the immunogen for the Glycophorin A antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
GYPA/280
Applications
IHC-P, WB
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the Glycophorin A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Glycophorin A antibody is available for research use only.
Storage Conditions
Store the Glycophorin A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Cytoplasmic, membranous
Image Legend
IHC-P: Formalin-fixed, paraffin-embedded human angiosarcoma stained with Glycophorin A antibody (clone GYPA/280) .
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