ABO Antibody / Blood Group Antigen H Type 2
Recognizes the blood group H type 2 antigens, trisaccharide Fuc (a1-2) Gal (b1-4) GlcNAc (b1) of human origin. This protein is the basis of the ABO blood group system. The histo-blood group ABO involves three carbohydrate antigens: A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of UDP-GalNAc) or to the B antigen (by addition of UDP-Gal), whereas O individuals lack such activity. It is expressed on endothelial cells, epithelial cells and granulocytes. Increased expression of this antigen has been observed on some tumor tissues such as gastric carcinomas, urothelial carcinomas, and colon carcinomas.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunofluorescence: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT
UniProt
P16442
Host
Mouse
Reactivity
Human
Immunogen
Clonality
Monoclonal
Isotype
IgM k
Clone
19-OLE
Applications
IF, IHC-P
Purity
PEG precipitation
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the ABO antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This ABO antibody is available for research use only.
Storage Conditions
Store the ABO antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Location
Cell surface
Image Legend
IHC: Formalin-fixed, paraffin-embedded human colon carcinoma stained with Blood Group Antigen H Type 2 antibody (19-OLE)
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