TOX-3 Antibody

It recognizes an ~63 kDa protein, which is identified as TOX3. It contains a high mobility group (HMG) -box, which regulates Ca2+-dependent transcription in neurons through interaction with the cAMP-response-element-binding protein (CREB) . TOX3 appears to be associated with breast cancer susceptibility and is expressed downstream of a cytoprotective cascade together with CITED1, a transcriptional regulator that does not bind directly to DNA. TOX3 is predominantly expressed in the brain and forms homodimers. TOX3 overexpression protects neuronal cells from cell death caused by endoplasmic reticulum stress or BAX overexpression through the induction of anti-apoptotic transcripts and repression of pro-apoptotic transcripts.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA (order BSA/sodium azide-free format for coating), Western blot: 1-2 µg/mL

UniProt

O15405

Host

Mouse

Reactivity

Human

Immunogen

A recombinant fragment (139 amino acid residues around aa 200-400) from the human protein was used as the immunogen for the TOX-3 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

TOX3/1124

Applications

ELISA, WB

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the TOX-3 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This TOX-3 antibody is available for research use only.

Storage Conditions

Store the TOX-3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the TOX-3 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Location

Cytoplasmic & nuclear

Image Legend

Western blot analysis of 1) recombinant partial protein, 2) A549 and 3) A431 stained with TOX-3 antibody (clone TOX3/1124) . Predicted molecular weight ~63 kDa.