Glypican 3 Antibody
Glypican-3 (GPC3) is a glycosylphospatidyl inositol-anchored membrane protein, which may also be found in a secreted form. Anti-GPC3 has been identified as a useful tumor marker for the diagnosis of hepatocellular carcinoma (HCC), hepatoblastoma, melanoma, testicular germ cell tumors, and Wilms tumor and hepatoblastoma, with a low or undetectable expression in normal adjacent tissue. In patients with thyroid cancer, expression of GPC3 is dramatically enhanced in certain types of cancers: 100% in follicular carcinoma and 70% in papillary carcinoma. Expression of GPC3 in follicular carcinoma is significantly higher than that of follicular adenoma. In contrast, GPC3 is not expressed in anaplastic carcinoma.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 5ul/test/million cells in 0.1ml, Immunofluorescence: 1-2 µg/mL
UniProt
P51654
Host
Mouse
Reactivity
Human
Immunogen
A recombinant fragment containing amino acids 511-580 from the human protein was used as the immunogen for the Glypican-3 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
SPM595
Applications
FACS, IF
Purity
Protein G affinity chromatography
Format
CF555 Conjugate
Buffer
500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the Glypican 3 antibody at 2-8oC, protected from light.
Limitations
This Glypican 3 antibody is available for research use only.
Storage Conditions
Store the Glypican 3 antibody at 2-8°C, protected from light.
Formulation
500 ul at 0.1 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the Glypican 3 antibody should be determined by the researcher.
Location
Cytoplasmic
Image Legend
Glypican 3 antibody (clone SPM595) used to staining PFA-fixed human HepG2 cells.
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