EMI1 Antibody
Early Mitotic Inhibitor-1 regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC) . It is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, EMI1 is transcriptionally induced by the E2F transcription factor. Overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb) . Depleting cells of EMI1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL for 60 min at RT, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
Q9UKT4
Host
Mouse
Reactivity
Human
Immunogen
A recombinant fragment (203 amino acid residues between aa 1-250) from the human protein was used as the immunogen for the EMI1 antibody.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
EMI1/1176
Applications
WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the EMI1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This EMI1 antibody is available for research use only.
Storage Conditions
Store the EMI1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Nuclear
Image Legend
IHC: Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with EMI1 antibody (clone EMI1/1176) .
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