SSEA-1 Antibody
CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on >95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells and some epithelial cells. CD15 antibody is very useful in the identification of Hodgkin s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P22083
Host
Mouse
Reactivity
Human
Immunogen
Myelomonocytic leukemia cells were used as the immunogen for the SSEA-1 antibody.
Clonality
Monoclonal
Isotype
IgM k
Clone
SPM490
Applications
IF, IHC-P
Purity
PEG precipitation
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Store the SSEA-1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This SSEA-1 antibody is available for research use only.
Storage Conditions
Store the SSEA-1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
Optimal dilution of the SSEA-1 antibody should be determined by the researcher.
Location
Cell surface and granular paranuclear
Image Legend
Immunofluorescent staining of human U937 cells with Calponin antibody (clone SPM490, green) and Reddot nuclear stain (red) .
Frequently Asked Questions
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