FUT4 Antibody
CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on >95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells and some epithelial cells. CD15 antibody is very useful in the identification of Hodgkin's disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 0.5-1ug/million cells, Immunofluorescence: 0.5-1 µg/mL
UniProt
P22083
Host
Mouse
Reactivity
Human
Immunogen
Myelomonocytic leukemia cells were used as the immunogen for the FUT4 antibody.
Clonality
Monoclonal
Isotype
IgM k
Clone
FR4A5
Applications
FACS, IF
Purity
PEG precipitation
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the FUT4 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This FUT4 antibody is available for research use only.
Storage Conditions
Store the FUT4 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the FUT4 antibody should be determined by the researcher.
Location
Cell surface and granular paranuclear
Image Legend
Immunofluorescent staining of human U937 cells with CD15 antibody (clone FR4A5, green) and Reddot nuclear stain (red) .
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