PLAP Antibody / Placental Alkaline Phosphatase
Reacts with a 70kDa membrane-bound isozyme (Regan and Nagao type) of placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is highly specific for PLAP and shows no cross-reaction with other isozymes of alkaline phosphatase. Anti-PLAP reacts with germ cell tumors and can discriminate between these and other neoplasms. Somatic neoplasms e.g. breast, gastrointestinal, prostatic, and urinary cancers may also immunoreact with antibodies to PLAP. Anti-PLAP positivity in conjunction with anti-keratin negativity favors seminoma over carcinoma. Germ cell tumors are usually anti-keratin positive, but they regularly fail to stain with anti-EMA, whereas most carcinomas stain with anti-EMA. Anti-PLAP has been useful in the diagnosis of gestational trophoblastic disease.
Product Specifications
CAS Number
9007-83-4
Specifications
UniProt
P05187
Host
Mouse
Reactivity
Human
Immunogen
Recombinant full-length human ALPP/placental alkaline phosphatase protein was used as the immunogen for the PLAP antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
SPM593
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*
Reconstitution
Store the PLAP antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This PLAP antibody is available for research use only.
Storage Conditions
Store the PLAP antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*
Location
Cytoplasmic and cell surface
Image Legend
IHC: Formalin-fixed, paraffin-embedded human placenta stained with PLAP antibody (clone SPM593) .
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