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Fibronectin Antibody

Epitope of this mAb is located in the 8th type III repeat in the cell-binding region of fibronectin. Fibronectins are disulfide-linked, dimeric glycoproteins of ~440kDa. They possess at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Fibronectins are involved in cell adhesion, tissue organization, and wound healing. Fibronectins are present in basement membranes, interstitial connective tissue matrix, and blood. Cellular fibronectin is widely distributed in the stroma of many malignant tumors. This mAb is excellent for staining of formalin-fixed, paraffin-embedded tissues.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P02751

Host

Mouse

Reactivity

Human

Immunogen

High molecular weight proteins secreted by cultivated human fibroblasts were used as the immunogen for the Fibronectin antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

568

Applications

FACS, IF, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the Fibronectin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Fibronectin antibody is available for research use only.

Storage Conditions

Store the Fibronectin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Connective tissue matrix

Image Legend

IHC: Formalin-fixed, paraffin-embedded human renal cell carcinoma stained with Fibronectin antibody (clone 568) .

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